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PhD student on In Vivo Dissection of Nucleotide Excision Repair

  • 49.14.18.TBW
  • 36 hours per week
  • Moleculaire Genetica

Job description

Nucleotide Excision Repair (NER) is a versatile DNA repair pathway that preserves genomic integrity by removing different types of DNA-helix distorting lesions. Hereditary defects in NER are associated with distinct, clinically heterogeneous diseases characterized by developmental failure, neurological defects, cancer predisposition and segmental progeria. Remarkably, in patients, tissues are affected differentially by distinct NER-deficiencies, which is currently not understood. In this project, we will utilize the model organism C. elegans to study the biological relevance and activity of NER in vivo at the molecular level, to better understand tissue-dependent organization of NER activity and pathogenesis of NER diseases. As NER protects against cancer and aging, studying NER function in different cell types is crucial to better understand human health.

The PhD candidate will focus on the development and utilization of C. elegans as in vivo tool, to dissect NER activity in the context of a complete organism and to understand the biological impact of NER-deficiency. To this end, the PhD candidate will employ a multidisciplinary approach, using various DNA repair assays, CRISPR-Cas9 technology, genetics, live confocal imaging, quantitative proteomics and transcriptomics

Work environment

The Erasmus MC stands for a healthy population and excellence in healthcare. By conducting groundbreaking work, we aim to push boundaries through leading the way in research, education and healthcare. We have access to the latest equipment and techniques in a state-of-the-art environment.

The laboratory of Prof. W. Vermeulen at the Department of Molecular Genetics performs innovative fundamental and applied research on genome stability. The group uses a multidisciplinary approach to advance understanding of the molecular mechanisms that protect our genome, working from the molecular genetic, biochemical and cell biological level to the level of intact eukaryotic organisms and patients. The group, composed of 13 international researchers, works in a highly collaborative and integrative manner with a strong focus on live-cell imaging, genetic and proteomic tools. Our group acts internationally at the forefront of the DNA repair field and offers a dynamic, challenging, and cooperative research and training environment.

Our laboratory offers an excellent training and supervision platform through a dedicated teaching program of our Graduate school Biomedical Sciences.

Qualifications and skills

We are seeking a highly motivated and talented candidate that holds a MSc degree in Biomedical Science, Molecular/ Cellular Biology, Biochemistry or related field with a basic knowledge of DNA repair pathways. Experience with standard molecular biology techniques, live cell imaging, proteomics and/or C. elegans will be a strong advantage. Excellent writing and presentation skills in English are recommended. As you will work in a dynamic international research environment, we need a team-player who is able to combine independent thinking with a collaborative attitude.

Being able to present a certificate of good conduct, a valid proof of identity, diploma's and/ or relevant registration such as BIG/ RGS are conditions for the appointment.
 

Terms of employment

You will receive a temporary position for 4 years. The gross monthly salary is € 2.336,- in the 1st year and increases to € 2.992,- in the 4th year (scale OIO). The terms of employment are according to the Collective Bargaining Agreement for Dutch University Medical Centers (CAO UMC).

Information and application

For more information about this position, please contact Dr. Hannes Lans, Department of Molecular Genetics, phone number: +31 (0)10 703 8169 or e-mail: w.lans@erasmusmc.nl. For more information about our research, visit www.vermeulenlab.com.  For queries regarding your application, please contact Jerry Chandansingh, Recruiter, phone number: +31 (0)10 704 49 83.

If you are excited by the thought of this position and would like to apply, please do so by using the application form on our website.
 

No agencies please.