Neurodevelopmental disorders are severe conditions impacting the life of patients and families. In the Barakat lab at the department of Clinical Genetics we investigate the role of gene regulation in these disorders, providing new insights for diagnostics, understanding of the disease mechanisms and future therapy development.
Our new project, funded by a ZonMw PSIDER grant, is focused on understanding how certain variants in a chromatin modifier gene are contributing to brain development and neural function and what are the cellular events behind the pathology of the disorder caused by these variants.
As a research assistant you will be involved in establishing and maintaining advanced induced pluripotent stem cell (iPSC)-derived neural cultures (neurons, brain organoids); study their functionality (electrophysiology) and gene expression profiles. You will work with iPSC, human embryonic stem cells and modify them with genetic engineering tools (CRISPR/Cas9, base editing). For the downstream analysis we will use (single-cell)RNA-seq, methylation profiling, immunofluorescent imaging, multi-electrode arrays etc. You will work with a postdoc and a PhD student on the main project and be involved in overall research activities of the group. For graduated MSC students, using this temporary position as a bridge to a follow-up PhD project could be an option.
(https://www.erasmusmc.nl/en/research/groups/barakat-lab-non-coding-genome-in-clinical-genetics) . We offer a welcoming and collaborative environment, where we work together for a common goal and where your contributions are recognised. The department of Clinical Genetics at the Erasmus MC is a leading institute for excellent genetic research on several forms of hereditary diseases, with a special focus on understanding basic molecular disease mechanism enabling future treatment of genetic diseases. A leading focus in this is on neurogenetic disorders including epilepsy (with the research groups of Mancini, Bonifati, Willemsen, Barakat, and Van Ham). We combine the use of patient materials, in vivo models (zebrafish, mouse) with state-of-the-art molecular and genetic approaches (next-generation-sequencing, epigenome-profiling, biomics, metabolomics).
An overview of some of our department’s research activities can be found at https://youtu.be/ifoF6y4rqIs . Our laboratory is equipped with all required facilities and equipment and can also make use of Erasmus MC Core-facilities (e.g. FACS, Cancer-treatment screening facility (CTSF), iPSC-, animal facility). Our department is embedded within the Pediatric Brain Centre (Sophia Children’s Hospital), is an NFU registered expertise center for Brain malformations, within the expertise center ENCORE, which is partner of ERN ITHACA, the European Reference network on rare congenital malformations (http://www.ernithaca.org/#). Our department has further strong collaborative bonds to the Erasmus MC departments of Cell Biology, Biochemistry, and Neuroscience, including various shared weekly scientific meetings.
Qualifications and skills
- You are highly motivated candidate with HBO/master’s degree in Biology, Biomedical sciences or related disciplines.
- You have a can-do attitude and are organised in your experimental work.
- You are experienced with cell culture and iPSC-work (preferably differentiation to neural cultures, brain organoids).
- You should be fluent in English (the working language in the lab).
Before you apply please check our conditions for employment.
For more information about this position, please mail Dr. Stefan Barakat, Principal Investigator, firstname.lastname@example.org or Dr. Kristina Lanko, postdoctoral researcher, email@example.com
In case of other requirements to apply:
Your application should include: motivation letter, Curriculum Vitae, list of courses and obtained grades during BSc and/or MSc program, a publication list, and a minimum of two reference letters.
If you are excited by the thought of this position and would like to apply, please do so by using the application form on our website.
No agencies please.